| External factors: | Nutlin-3a |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experimental category: | HL |
| Tissue type: | -- |
| Cell name: | Fibroblast,GM08402,NHF-hTERT,IMR-90,MRC-5 |
| PMID: | 18451145 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | A high percentage of cells that had been treated with nutlin-3a were stained positive for the low pH β-gal,indicating cells had undergone senescence.All three cell strains showed a morphologically senescent phenotype after nutlin-3a treatment. Again, almost 100% of the cells showed positive staining. |
| Target gene: | P53//MIR-34A//MIR-34B//MIR-34//ING2 |
| R-EF-Target gene: | Activation//Upregulation//Upregulation//Upregulation//Downregulation |
| Official symbol(s): | P53//MIR-34A//MIR-34B//MIR-34//ING2 |
| Target gene experiment: | RT-PCR//qRT-PCR//Western blot |
| Target gene description: | In NHF-hTERT cells, the total amount of p53 expression was increased by nutlin-3a. In contrast, there was no increase of p53 expression with nutlin-3a treatment in the p53 null cells.mir34a, mir34b, and mir34c levels were shown to be up-regulated to varying amounts in various human tumor cell lines that were wild type for p53 after treatment with nutlin-3a after 24 hours. Interestingly, ING2 expression in the cells treated with nutlin-3a was remarkably decreased compared with nontreated cells while ING2 expression was not changed in nutlin-3b–treated cells.ING2 mRNA transcripts were consistently decreased by nutlin-3a treatment in all cell lines using a real-time PCR method. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: