| External factors: | Scopoletin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | MTT assay//SA-β-gal activity assay |
| Description: | The effect of scopoletin on the viability of IMR 90 cells were measured using MTT assay.Scopoletin at 16μM or below did not show cytotoxicity.Representative images of SA-β-Gal staining in control group (H2O2 treatment). The number of SA-β-gal positive cells in control groups was increased in a dose dependent manner.The quantification of SA-β-Gal staining level. While SA-β-Gal staining was increased with the concentration of hydrogen peroxide, scopoletin significantly inhibited the SA-β-Gal staining stimulated by hydrogen peroxide in IMR 90 cells. |
| Target gene: | P53//NF-κB//NRF2//P-FOXO1 |
| R-EF-Target gene: | Activation//Downregulation//Upregulation//Upregulation |
| Official symbol(s): | P53//NFKB1//NRF2//P-FOXO1 |
| Target gene experiment: | Western blot |
| Target gene description: | Scopoletin treatment significantly increased the expression level of p53. However, the levels of p-p53 and acetyl-p53, Activate forms of p53, expressions were decreased in the presence of scopoletin compared with blank group. Furthermore,the expression level of p21 having the DNA binding site of p53 in its promoter was decreased in similar to the expression pattern of Activate p53. These results indicate that scopoletin promotes activation of p53 in human lung fibroblast.It was found that the protein expression levels of p50 and p65, a dimer of NF-κB associated with inflammation, were reduced compared with blank group. In contrast, the expression levels of Nrf-2 and p-FoxO1 modulating the expression of superoxide dismutase in nucleus were enhanced in the presence of scopoletin compared with blank group in IMR 90 cells. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: