| External factors: | Pigment epithelium-derived factor |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Most of the normoxic cultured-cells were positive for SA-β-gal after ceasing to proliferate,whereas few were stained in the other culture at the same time, which was a highly significant difference. |
| Target gene: | P53//P16 |
| R-EF-Target gene: | Downregulation//Downregulation |
| Official symbol(s): | P53//P16 |
| Target gene experiment: | Western blot |
| Target gene description: | Consistent with previous findings, the levels of p53 and p16 increased with the lifespan of normoxic cultured-MSCs. In contrast, PEDF lowered the overall transcript level and protein expression of p53 and p16 by Day 95. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: