| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Immunostaining//SA-β-gal activity assay |
| Description: | We found that the number of Ki-67 positive cells was dramatically decreased after H2O2 induction, indicating a significant decrease in the proliferation rate;The cells were treated with 100 μM H2O2 for 2 h and SA-β-gal staining was performed. It was noted that, following H2O2-induced senescence, the percentage of SA-β-gal positive cells increased significantly. |
| Target gene: | P53//P21 |
| R-EF-Target gene: | Upregulation//Upregulation |
| Official symbol(s): | P53//P21 |
| Target gene experiment: | Semi-qPCR//qPCR |
| Target gene description: | Cell cycle marker genes such as p21 and p53 were initially upregulated post H2O2 treatment. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: