External factors: | H2O2 |
Aging type: | Accelerate |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Aging |
Experiment: | Immunostaining//SA-β-gal activity assay |
Description: | We found that the number of Ki-67 positive cells was dramatically decreased after H2O2 induction, indicating a significant decrease in the proliferation rate;The cells were treated with 100 μM H2O2 for 2 h and SA-β-gal staining was performed. It was noted that, following H2O2-induced senescence, the percentage of SA-β-gal positive cells increased significantly. |
Target gene: | P53//P21 |
R-EF-Target gene: | Upregulation//Upregulation |
Official symbol(s): | P53//P21 |
Target gene experiment: | Semi-qPCR//qPCR |
Target gene description: | Cell cycle marker genes such as p21 and p53 were initially upregulated post H2O2 treatment. |
Regulatory pathway: | -- |
R-EF-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation: