| External factors: | Curcumin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | We tested the influence of this compound in human breast cancer MCF 7 cell line and human osteosrcoma U2OS cell line. Both cell lines underwent cellular senescence as revealed by increased activity of SA-β-gal as well as the induction of the p53 and p21 proteins detected in Western blot analysis. |
| Target gene: | P53//P21 |
| R-EF-Target gene: | Upregulation//Upregulation |
| Official symbol(s): | P53//P21 |
| Target gene experiment: | Western blot |
| Target gene description: | Induction of cellular senescence was further confirmed by examination of the protein level. We detected an increased level of the p53 (only in HCT116 p53+/+ cells) and p21 proteins. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: