| External factors: | Resveratrol |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Colorectal cancer |
| Experiment: | SA-β-gal activity assay//BrdU assay//Cell morphological analysis |
| Description: | 30 M RV slowed down proliferation until cells finally came to a complete growth stop after 30 40 days of treatment. Cells that were growtharrested for at least 10 days were stained for SA-gal expression and showed up to 60% positive staining, indicating that 30 M RV induces a senescent phenotype in HCT 116 colon carcinoma cells. RV (30 M)-treated cells displayed a flattened and enlarged morphology and an abrogated bromodeoxyuridine-labeling index ( 5%). |
| Target gene: | P53//P21//P38MAPK//ATM |
| R-EF-Target gene: | --//--//--//Activation |
| Official symbol(s): | P53//P21//P38MAPK//ATM |
| Target gene experiment: | SA-β-gal activity assay//Western blot//Knockdown |
| Target gene description: | However, staining for SA-β-gal expression revealed a significantly lower percentage of positive cells in the knock-out lines. For unknown reasons, the p53 clones of the HCT line displayed an overall weaker expression of SA-β-gal (up to 60% positive cells in p21WTversus up to 25% cells in p53 WT cells after the same RV treatment protocol).Furthermore, the growth arrest in p53 and p21 knock-out cells was reversible after withdrawal of RV, whereas halted WT cells were not able to resume proliferation upon RV removal. In accordance with the elevated ROS levels, the redox-sensitive protein kinase MAPK-p38 was found to be significantly.We could demonstrate that both inhibitors overcame RVtriggered p53 phosphorylation (Ser-15) and p21 induction. Moreover, long term incubation with RV and KU55993 was able to significantly lower the number of senescent (SA-gal-positive) cells when compared with RV alone. hyperphosphorylated 24 h after treatment with RV without changes in its total levels. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: