| External factors: | Methoxyamine |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Colorectal cancer |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | Examined senescence-activated β-gal activity following these treatments. Senescence-activated β-gal activity was examined on days 5 to 8 following ionizing radiation and was found to be positive in IUdR- and IUdR/methoxyamine–pretreated cells. Spot senescence-activated β-gal positive cells were also found in the control and methoxyamine-pretreated cells;During the course of the experiments, we noted that after ionizing radiation, IUdR- and IUdR/methoxyamine–pretreated cells exhibited senescence-like morphologic features . |
| Target gene: | P53//P21//RB |
| R-EF-Target gene: | Upregulation//Upregulation//Activation |
| Official symbol(s): | P53//P21//RB |
| Target gene experiment: | Western blot |
| Target gene description: | Indeed, both accumulation of p53 and induction of p21, the major mediator of p53-dependent senescence, increase more significantly in IUdR/methoxyamine–pretreated cells at both early and later times following ionizing radiation. Furthermore, IUdR/methoxyamine–pretreated cells show reduced hyperphosphorylated Rb(S780) (inActivate form) but greater hypophosphorylated Rb (Activate form) compared with IUdR-pretreated cells . |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: