| External factors: | Aldosterone |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | An increase in SA-β Gal staining, which is typically observed in senescent cells, was detected in the kidney, particularly the proximal tubules, of aldosterone-infused rats,but not in vehicle-infused rats. Aldosterone (1 and 10 nmol/liter for 3 d)dose-dependently increased the percentage of SA-β Gal–expressing HPTCs compared with vehicle treatment in both TSIm-HPTCs and N-HPTCs . |
| Target gene: | P53//P21//SIRT1 |
| R-EF-Target gene: | Upregulation//Upregulation//Downregulation |
| Official symbol(s): | P53//P21//SIRT1 |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | Aldosterone infusion markedly increased the expression of p53 mRNA and protein.Aldosterone strongly increased the expression of p21 mRNA and protein.On the other hand, the gene expression of SIRT1, an enzyme that inactivates p53 by deacetylation, was reduced in aldoste-rone-infused rats compared with that in vehicle-infused rats. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: