| External factors: | Cigarette smoke extract |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Furthermore, we performed histochemical analysis of senescence associated-β-galactosidase (SA-β-gal) activity and found that Beas2b cells exposed to 10%-CSE exhibited significant (p<0.001) increase in SA-β-gal-positive (blue) cells in comparison to air-exposed or cysteamine-treated cells. |
| Target gene: | P53//SIRT1 |
| R-EF-Target gene: | Downregulation//Downregulation |
| Official symbol(s): | P53//SIRT1 |
| Target gene experiment: | Western blot |
| Target gene description: | We observed a decrease in p53 in soluble protein fractions of 10%-CSE treated cells that is indicative of a reduction in DNA damage recognition and repair. Abrogation of Sirt1 was observed in the soluble protein fractions prepared from Beas2b cells upon exposure to 10% CSE in comparison to air-exposed cells; the loss of Sirt1 is indicative of cell cycle arrest and cellular senescence. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: