| External factors: | UVB |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry//PI staining//Annexin V binding assay |
| Description: | This was termed senescence-associated betagalactosidase or SA-β-gal. Our study revealed strong activity of SA-β-gal in UVB-irradiated HDFs, which, as one of the biomarkers of senescence, indicated that cells were induced to a senescencelike state by UVB exposure. As shown by PI staining and the annexin V method, we found UVB irradiation caused cell cycle arrest, with most HDFs being arrested on G0/G1 phase, and it might play a key role in aging process by initiating cell aging. |
| Target gene: | P66SHC//FKHRL1 |
| R-EF-Target gene: | Upregulation//Upregulation |
| Official symbol(s): | P66SHC//FOXO3 |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | p66Shc and FKHRL1 were significantly up-regulated by UVB irradiation. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: