| External factors: | H2O2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | Flow cytometry//SA-β-gal activity assay |
| Description: | To confirm the features of senescence, cell cycle distribution was analyzed in H2 O2 treated TIG-3 cells. As compared to control cells, 150 M H2O2 increased the population of G1 phase cells from 52.9 0.52% to 69.0 1.23% and decreased the population of S phase cells from 32.7 0.29% to 15.4 3.04% at 24h after treatment. These results suggest that 150 M H2 O2 inhibited cell cycle progression at the G1 phase.48h after the addition of H2O2,79.4 12.2% of treated cells were SA-β-gal positive,whereas 1.7±1.2% of control cells were SA-β-gal positive . |
| Target gene: | PARP//SIRT1//P53 |
| R-EF-Target gene: | --//--//-- |
| Official symbol(s): | PARP1//SIRT1//P53 |
| Target gene experiment: | Measurement of intracellular NAD+ level//SDS-PAGE//Western blot |
| Target gene description: | The depletion of intracellular NAD+ levels induced by 150 μM H2O2 treatment was significantly inhibited by the addition of ANI, a PARP inhibitor. The expression of acetylated p53 increased by 4h and continued to increase for up to 24h, while p53 expression increased up to 12h. The expression of p21, which is a transcriptional target of p53, was increased at 12h and continued to increase for up to 24h. On the other hand, p53 acetylation was increased by the addition of nicotinamide, a SIRT1 inhibitor. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: