| External factors: | Adriamycin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | Adr but not Etop induced cellular senescence . |
| Target gene: | PERK//P53//RKIP |
| R-EF-Target gene: | Downregulation//Upregulation//Upregulation |
| Official symbol(s): | EIF2AK3//P53//RKIP |
| Target gene experiment: | Western blot |
| Target gene description: | As the A549 cell line is p16/p14 negative, these results suggested that Adr induced cellular senescence in a p16/Rb-independent manner. In contrast to reduction of p-ERK and increase of SA-β-Gal positive cells in response to Adrin p53-positive cell lines, p53-null cells (HCT116 p53 -/- ) did not show the reduction of p-ERK and senescence.However, reconstruction of p53 in HCT 116 p53-/-cells by transfection of wild-type p53 could restore the Adr-induced senescence. As we expected, Adr treatment induced the RKIP expression along with suppression of p-ERK . Previously, we showed that reduction of p-ERK showed the dependence with p53, and RKIP was induced by Adr. |
| Regulatory pathway: | -- |
| R-EF-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation: