| External factors: | Lactoferrin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | SA-β-galactosidase staining revealed that lactoferrin suppressed the hydrogen peroxide-induced senescence of hMSCs.Lactoferrin suppressed the hydrogen peroxide-induced cell death of hMSCs in a dose-dependent manner. |
| Regulatory pathway: | Akt |
| R-EF-Pathway: | Activation |
| Official symbol(s): | AKT1 |
| Pathway experiment: | Western blot//Fluorescence microscopy |
| Pathway description: | Our results showed that pretreatment with lactoferrin increased the hydrogen peroxide-induced phosphorylation of Akt compared with only hydrogen peroxide treatment. In addition, treatment of PI3K/Akt inhibitor, Wortmannin decreased the lactoferrin-induced inhibition of ROS generation or cell survival in hydrogen peroxide-treated hMSCs. |
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