| External factors: | AGEs |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Diabetes kidney disease |
| Experiment: | Immunostaining//SA-β-gal activity assay//SAHF//PI staining |
| Description: | Double immunofluorecent staining demonstrated that p16 positive cells were colocalized in the AGE-positive cells.P16-siRNA considerably reduced the ratio of SA-β-gal and SAHF-positive RTECs as well as the proportion of RTECs in the G0/G1 phase in AGE-BSA treated group . |
| Regulatory pathway: | ATF4-p16 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | ATF4-CDKN2A |
| Pathway experiment: | Immunostainings//Western blot |
| Pathway description: | Linear correlation analyses showed that nuclear ATF4 expression was positively correlated with AGE deposition.The expression of ATF4 and p16 was co-localized in the nucleus of the same SAHF positive cells . ATF4 siRNA resulted in a significant decrease in AGE-induced p16 expression and premature senescence of PTECs. |
Annotation: