| External factors: | Pterostilbene |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Lung cancer |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Western blot |
| Description: | Consistent with the mild inhibition of cell growth, treatment of A549 cells with relatively low concentrations of PT (5 and 10 μM) in the presence of 10% serum did not perturb cell cycle progression at 24 hr. However, treatment of A549 cells with the same concentration of PT in the absence of serum induced S-phase arrest .cell senescence was well induced by 72 hrs treated PT in HBECR cells than in HBECR/p53i cells, in a dose dependent manner in two different assays.A senescence inducer, p21 and p53 amount were increased in HBECR. |
| Regulatory pathway: | ATM-ATR-CHK1-p53 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | ATM-ATR-CHK1-TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | The ATM/ATR-CHK1/2 axis is activated upon treatment with low doses of PT, an effect that is indicative of replication stress. |
Annotation: