| External factors: | MLN8237 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Melanoma |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Cytokine array |
| Description: | Indeed, after 5 days of treatment in vitro,we observed that the cellular size was greatly enlarged ,which is a characteristic associated with senescence.β-galactosidase activity was evaluated and found to be enhanced in drug-treated Hs294T cells and in other melanoma cell lines (SK-Mel-2, SK-Mel-5 and SK-Mel-28).The results demonstrated that IL-6, IL-7, IL-10, GM-CSF, IL-8, RANTES, GRO and GRO-a were upregulated in response to drug treatment. |
| Regulatory pathway: | ATM-Chk2//NF-kB |
| R-EF-Pathway: | Activation//Activation |
| Official symbol(s): | ATM-CHEK2// |
| Pathway experiment: | SA-β-gal activity assay//Knockdown//Luciferase reporter assay |
| Pathway description: | We knocked down either ATM or Chk2 and found that senescence was reduced >30% in knockdown cells,indicating that the ATM/Chk2 pathway mediates MLN8237-induced senescence.An NF-kB luciferase assay was also performed using Hs294T NF-kB reporter cells. NF-kB transcriptional activity was significantly increased after treatment with 1mM MLN8237 for 5 days . |
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