| External factors: | γ-irradiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Chronic c-irradiation efficiently induced cellular senescence and 0.694 mGy/min was more potent than 0.347 mGy/min in this assay. This result was consistent with results obtained using the colony survival assay. |
| Regulatory pathway: | ATM-p53-p21 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | ATM-CDKN1A-TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | TP53 or p21 levels were knocked down in BJ1/hT fibroblasts. This treatment abolished the cell-proliferation inhibition induced by chronic c-irradiation .The ATM inhibitor KU55933 inhibited the phosphorylation of TP53 and checkpoint kinase 2 (CHEK2) (ATM kinase substrates) and reduced p21 induction in response to c-irradiation. |
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