| External factors: | Copper |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Glioblastoma |
| Experiment: | SA-β-gal activity assay//Cell viability assay//MTT assay |
| Description: | SA-β-gal activity assay:U87-MG cells exposed to 250 or 500 μM CuSO4 presented an enlarged cellular volume and altered shape, resembling the typical senescent phenotype. Cell viability assay:With increasing concentrations of copper sulfate, the cell viability was accordingly decreased . MTT assay:the 250 or 500 μM copper sulfate group showed a dramatic decrease in cell proliferation when compared with the control group. |
| Regulatory pathway: | Bmi-1 |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | BMI1 |
| Pathway experiment: | Western blot//RT-PCR |
| Pathway description: | CuSO4-treated cells (250 μM) showed increased protein levels of p21 and p16, but a decreased level of Bmi-1, when compared with the control.Cells exposed to 250 μM CuSO4 showed statistically significant overexpression of ApoJ,TGF-β1,p21 and p16 (4.0-, 1.7-, 3.2- and 2.4-fold, respectively) but a marked decrease in Bmi-1. |
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