| External factors: | Dihydroartemisinin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Hepatocellular carcinoma |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | To further explore the underlying mechanisms of DHA on HepG2.2.15 cells, cellular senescence was detected by senescence-associated-β-Galactosidase assay. And the associated protein expression was measured by western blot.Exposure of HepG2.2.15 cells to DHA for 48 hours increased the senescent cells compared with the control. |
| Regulatory pathway: | DDR//Akt-mTOR |
| R-EF-Pathway: | --//-- |
| Official symbol(s): | DDR1//AKT1-MTOR |
| Pathway experiment: | Western blot |
| Pathway description: | Compared with cells treated with vehicle, the p-ATM, p-ATR, γ-H2AX, P53 and P21 were elevated while the telomere shelterin TPP1 was significantly down-regulated in cells treated with DHA. These results demonstrated that DHA might induce HepG2.2.15 cellular senescence through DDR pathway.In the present study, some key molecules in AKT-mTOR signaling were also detected by western blot. The p-AKT, p-mTOR, p-p70S6K, p-4EBP1 were down-regulated and followed by autophagy associated protein LC3, p-AMPK, p62 increased obviously. |
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