| External factors: | Busulfan |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//BrdU assay |
| Description: | The growth of WI38 cells was permanently arrested after 24 h treatment with BU,while control untreated cells proliferated exponentially.This suggestion is supported by the observation that cells briefly treated with BU exhibited a significant reduction in BrdU incorporation (BU, 3.6 ± 1.5% vs Ctrl, 45.1 ± 4.7%, P< 0.0001) and a drastic increase in SA-β-gal staining (BU, 75.4 ± 6.6% vs Ctrl, 9.7 ± 4.9%, P< 0.0001) as compared with control untreated cells at 11 days after the initial treatment . |
| Regulatory pathway: | ERK-p38 MAPK |
| R-EF-Pathway: | Activation |
| Official symbol(s): | MAPK1-MAPK14-MAPK |
| Pathway experiment: | Western blot//SA-β-gal activity assay//BrdU assay |
| Pathway description: | BU treatment incited a moderate p53 activation, but strong Erk,p38, and JNK phosphorylation, in a time-dependent manner.It was found that inhibition of Erk or p38 abrogated BU-induced increase in SA-β-gal staining and reduction in BrdU incorporation and cell proliferation in WI38 cells as compared with the cells treated with BU alone . |
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