| External factors: | Estrogen |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Osteoporosis |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | The role of estrogen on anti-senescence was verified by the decreased SA-β-gal positive cells and alleviated expression of senescence markers. |
| Regulatory pathway: | ERβ-SATB2 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | ESR2-SATB2 |
| Pathway experiment: | Western blot//qRT-PCR//Knockdown//Colony formation assay |
| Pathway description: | Higher expression levels of SATB2, Nanog, Sox2 and Oct4, were observed in BMSCs treated with estrogen relative to the control group.A reduction in colony formation was observed with both siRNA-ERβ and ICI182, 170 treatment. Consistent with reduced stemness, lower expression of Nanog, Sox2 and Oct4 was evident. In the siRNA-ERβ or ICI182, 170 groups, SATB2 expression decreased up to 0.5-fold, conversely, estrogen treatment induced SATB2 expression by up to 1-fold in BMSCs. Similar effects were also observed at the protein level. |
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