Aging Overview

External factors

External factors: Fucoidan
Aging type: Prevent
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: L
Tissue type: Adipose
Cell name: MSC
PMID: 29642406
Experiment: SA-β-gal activity assay//Cell cycle analysis
Description: A SA-β-gal assay showed that treatment with fucoidan significantly decreased MSC senescence caused by p-cresol exposure. Furthermore, cell cycle analysis revealed that fucoidan inhibited the decrease in proliferation caused by p-cresol.


Regulatory relationship

Regulatory pathway: FAK-Akt-TWST
R-EF-Pathway: Activation
Official symbol(s): FAK-AKT-TWST
Pathway experiment: Western blot//qPCR//SA-β-gal activity assay
Pathway description: We evaluated activation of the FAK-Akt-TWIST signal pathway after treatment with fucoidan (10 μg/mL) for 0, 24, 48, or 72 h. As expected, phosphorylation of FAK and Akt was significantly increased after fucoidan treatment in a time-dependent manner, with the maximal effect seen at 48 h. The expression of TWIST was also significantly increased in a time-dependent manner . In addition, Akt inhibition blocked the mRNA and protein expression of TWIST, indicating that fucoidan mediated TWIST expression is dependent on FAK-Akt phosphorylation.Our results show that the inhibition of Akt signaling increased the number of SA-β-gal positive cells and changed the cellular morphology of MSCs, suggesting that fucoidan-mediated Akt signaling is involved in the protection of p-cresol-induced MSC senescence.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view