External factors: | Fucoidan |
Aging type: | Prevent |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Aging |
Experiment: | SA-β-gal activity assay//Cell cycle analysis |
Description: | A SA-β-gal assay showed that treatment with fucoidan significantly decreased MSC senescence caused by p-cresol exposure. Furthermore, cell cycle analysis revealed that fucoidan inhibited the decrease in proliferation caused by p-cresol. |
Regulatory pathway: | FAK-Akt-TWST |
R-EF-Pathway: | Activation |
Official symbol(s): | FAK-AKT-TWST |
Pathway experiment: | Western blot//qPCR//SA-β-gal activity assay |
Pathway description: | We evaluated activation of the FAK-Akt-TWIST signal pathway after treatment with fucoidan (10 μg/mL) for 0, 24, 48, or 72 h. As expected, phosphorylation of FAK and Akt was significantly increased after fucoidan treatment in a time-dependent manner, with the maximal effect seen at 48 h. The expression of TWIST was also significantly increased in a time-dependent manner . In addition, Akt inhibition blocked the mRNA and protein expression of TWIST, indicating that fucoidan mediated TWIST expression is dependent on FAK-Akt phosphorylation.Our results show that the inhibition of Akt signaling increased the number of SA-β-gal positive cells and changed the cellular morphology of MSCs, suggesting that fucoidan-mediated Akt signaling is involved in the protection of p-cresol-induced MSC senescence. |
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