| External factors: | Thiostrepton |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Breast cancer |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | MCF-7 cells treated with thiostrepton exhibited SA-β-gal activity and their morphology changed, with the cells becoming enlarged and flattened.The SA-β-gal activity was determined to be increased in a dose-dependent manner, with the highest activity observed at 4 μM. MDA-MB-436 cells also exhibited senescence morphology, with positive SA-β-gal activity, after being exposed to thiostrepton. |
| Regulatory pathway: | FOXM1 |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | FOXM1 |
| Pathway experiment: | RT-PCR |
| Pathway description: | After 24 h of thiostrepton exposure, MCF-7 cells exhibited downregulation of FOXM1. |
Annotation: