| External factors: | Irradiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Dry-mouth syndrome |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//RT-PCR |
| Description: | Histological analysis performed over 12 weeks post-irradiation revealed a gradual increase in the appearance of large cells with notably enlarged, hyperchromatic nuclei, morphologically reminiscent of cells undergoing senescence (28).Salivary glands collected 8 weeks post-irradiation showed substantially increased SA-βgal activity located largely in ductal cells and also in some acini.Real-Time PCR analysis of salivary glands 2 and 8 weeks post-irradiation revealed strongly increased expression of the senescence-associated markers (31), p21, p19ARF, DcR2, and also the SASP genes, IL-6 and PAI-1, up to 21-fold. |
| Regulatory pathway: | IL-6 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | IL6 |
| Pathway experiment: | ELISA |
| Pathway description: | Analysis of serum IL-6 protein levels by ELISA revealed strong elevation in IL-6 expression that appeared roughly 6 hours post-irradiation and lasted less than one day . |
Annotation: