| External factors: | 27-hydroxycholesterol |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Neurodegenerative disease |
| Experiment: | SA-β-gal activity assay |
| Description: | In the present study, we demonstrated that 27HC induced apparent cellular senescence in nerve cells. Senescence-associated β-galactosidase(SA-β-Gal) assay revealed that 27HC induced senescence in both BV2 cellsand PC12 cells. |
| Regulatory pathway: | IL-6-STAT3 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | IL6-STAT3 |
| Pathway experiment: | DCFH assay//Western blot//ELISA |
| Pathway description: | Furthermore, we demonstrated that 27HC promoted the accumulation of cellular reActivate oxygen species (ROS) innervecells and subsequently activation of IL-6/STAT3 signaling pathway. Notably, treatment with the ROS scavenger N-acetylcysteine (NAC) markedly blocked 27HC-induced ROS production and activation of IL-6/STAT3. |
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