External factors: | Dehydroepiandrosterone |
Aging type: | Prevent |
Aging characteristic: |
Category: | Chemical compounds |
Phenotype: | Aging |
Experiment: | SA-β-gal activity assay//Western blot |
Description: | When we pre-treated with 50 nmol/L of DHEA for 1h prior to LA treatment,the number of SA-β-Gal-positive cells significantly decreased compared to the cells treated with LA alone.However,pre-treatment with DHEA substantially increased the protein level of ppRB(an indicator of senescence). |
Regulatory pathway: | JNK |
R-EF-Pathway: | Activation |
Official symbol(s): | MAPK8 |
Pathway experiment: | SA-β-gal activity assay//Western blot |
Pathway description: | After HAECs were pre-treated with the JNK inhibitor, the inhibitory effect disappeared. Similarly, the increased expression of ppRB by DHEA treatment was also abolished after JNK inhibition. |
Annotation: