| External factors: | PQQ |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Westen blot |
| Description: | Compared with HG group, the significantly increased of P16 and P21 could be reduced respectively in PQQ plus HG group. Compared with NG group, more SA-β-gal positive cells were observed in HG-induced HK-2 cells. However, PQQ treatment resulted in less SA-β-gal positive cells in comparison with the HG group. |
| Regulatory pathway: | Keap1-Nrf2 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | KEAP1-NFE2L2 |
| Pathway experiment: | qRT-PCR//Western blot//Immunofluorescence |
| Pathway description: | The levels of protein Nrf2, GPx-3, GST, HO-1 and NQO-1 were reduced in HK-2 cells exposed to HG for 48 h (P < 0.05). However, the expression of Nrf2 and downstream pathway proteins were increased post PQQ stimulation. In addition, we found that HG significantly increased the protein level of Keap1. However, the protein expression of Keap1 was inhibited by PQQ. PQQ treatment also enhanced HG-induced mRNA expression levels of Nrf2 . |
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