| External factors: | Melatonin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell counting |
| Description: | Indeed, treatment with melatonin significantly reduced the SA-β-gal activity in IMR90 cells cultured with media (OIS-CM) from H-RasV12transfected senescent IMR90 cells (65.5% in OIS-CM-treated group vs 20.2% in OIS-CM and melatonin-treated group, P<0.05). Consistent with the above observations, we also found that melatonin blocked the OIS-CM-induced growth arrest of IMR90 cells, as determined by focus formation assay (16.3% in OIS-CM-treated group vs and 64.1% in OIS-CM and melatonintreated group, P<0.05) and cell counting . We further observed that treatment with melatonin significantly reduced expression of OIS-CM-induced SASP genes (Illustrated by the case of IL8, 93.1-fold in OIS-CM-treated group vs 25.9-fold n OIS-CM and melatonin-treated group, P<0.05). |
| Regulatory pathway: | MacroH2A1 |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | MACROH2A1 |
| Pathway experiment: | Western blot |
| Pathway description: | Strikingly, melatonin treatment dramatically reduced macroH2A1 signaling during OIS. |
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