| Description: |
Treatment with Gyp-L significantly increased the percentages of SA-β-gal-positive cells .The proportion of EdU-positive cells was higher in the control group, which was remarkably reduced in a concentration-dependent manner in the presence of Gyp-L, indicating that Gyp-L inhibited the proliferative activity of cancer cells.we detected the senescence-associated expression of SASP, such as IL-1α, IL-6, TIMP-1, CXCL-1 and CXCL-2, by qRT-PCR. As expected, the mRNA expression levels of all cytokines were increased by Gyp-L.Flow cytometry assay results demonstrated that a progressive increase of cells, retardant in S-phase, occurred in hepatic and esophagus cancer cells when treated with different concentrations of Gyp-L.Gyp-L significantly reduced the expression of all cell cycle regulators, such as CDK2, CDK4, CDK6, and cyclin D1, which was consistent with the arrested cell cycle. |