| External factors: | KF866 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | The cells were flattened and enlarged,and the cell density was much smaller than that of control because of cell senescence. In addition, the result reveals that FK866 significantly and dosedependently induced SA-βG activity, as detected by the double-substrates method. |
| Regulatory pathway: | NAD+-SIRT1 |
| R-EF-Pathway: | Downregulation |
| Official symbol(s): | NAD-SIRT1 |
| Pathway experiment: | Acid extraction method//enzymatic cycling method |
| Pathway description: | FK866 significantly decreased intracellular NAD in a concentration- and timedependent manner.SIRT1 activity was estimated using the Ac-p53/p53 ratio during the 5-day incubation with F50. As we expected, the Ac-p53/p53 ratio increased by about 20 % during day 1 and day 4, with the highest level achieved on day 5. The results showed that SIRT1 activity was decreased by the treatment of F50. |
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