| External factors: | HFD |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Cardiovascular disease |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | In accordance with expectations,the HFD group showed a significant elevation in SA β-gal-positive areas of the endothelium (stained as blue) in comparison with the vehicle control group.A faint staining pattern of SMP30 was found in the endothelium (marked by CD-31) of the aortic roots in mice fed with HFD. |
| Regulatory pathway: | NLRP3 |
| R-EF-Pathway: | Upregulation |
| Official symbol(s): | NLRP3 |
| Pathway experiment: | Western blot |
| Pathway description: | The results of western blot analysis showed that D-gal significantly increased the expression of NLRP3 in HUVECs as compared with that of vehicle control. On the contrary, PSPC administration depressed the amplification of NLRP3 induced by D-gal. A similar pattern of NLRP3 was observed for the immunofluorescence activities in the brachiocephalic artery of mice which were fed with HFD or HFD+PSPC . |
Annotation: