| External factors: | Hypoxic |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis//EdU assay |
| Description: | The EdU incorporation rate was significantly higher in Hx-cultured hADMPCs than in Nx-cultured hADMPCs, suggesting that cell growth was increased in the Hx culture condition.Flow cytometry analysis revealed that ROS were generated at higher levels in hADMPCs when cultured in the Nx condition, suggesting that reduced production of ROS in the Hx condition may prevent the cells from entering replicative senescence.We also found that Nx-cultured hADMPCs were larger with a more irregular shape, which suggests that the Hx culture condition prevented hADMPCs from entering senescence [35]. To further investigate this phenomenon, cellular senescence was measured by staining for SA-β-Gal, which revealed that SA-β-Gal activity was increased in Nx-cultured hADMPCs at passage 17. |
| Regulatory pathway: | Notch |
| R-EF-Pathway: | Activation |
| Official symbol(s): | Notch1 |
| Pathway experiment: | Western blot//qPCR |
| Pathway description: | As expected, levels of cleaved NOTCH1, an activated form of NOTCH1, were significantly increased (greater than twofold) in the Hx culture condition. Q-PCR analysis revealed that HES1, a downstream target of Notch signaling, was upregulated in Hx-cultured hADMPCs, which also indicated that Notch signaling was activated in the Hx culture condition. |
Annotation: