| External factors: | Molecular hydrogen |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | The number of SA β-gal-positive cells was significantly higher at 24h after TCDD exposure (18.6±3.8% vs 5.5±1.4% SA β-gal-positive cells in the control). By contrast, in media with H2 and TCDD, the percentage of SA β-gal-positive cells was similar to that for the control, 5.5±1.9% . At 48 h after TCDD exposure, the percentage of SA β-galpositive cells was significantly higher than in the control (19.7±3.6% vs 5.2±1.3%), and the percentage of SA β-galpositive cells was 16.4±3.2% in TCDD-exposed cells cultured in hydrogen-rich medium, which indicates that, at 48h, H2 no longer suppressed the increase in SA β-gal-positive cells.Total p53 expression in HUVECs did not significantly differ between normal and hydrogen-rich media or between media with and without TCDD . Although acetyl-p53 expression was significantly higher at 24h after TCDD exposure than in the control, H2 significantly suppressed this increase in acetyl-p53 expression. However,at 48h after TCDD exposure, H2 did not suppress the TCDDinduced increase in acetyl-53 expression. |
| Regulatory pathway: | Nrf2 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | NFE2L2 |
| Pathway experiment: | Western blot |
| Pathway description: | We therefore investigated whether the antioxidant effects of H2 might depend on activation of Nrf2, using Western blot analysis to evaluate expression of phospho-Nrf2 and its antioxidant enzymes, HO-1 and NQO-1, after exposure of HUVECs to TCDD. In the H2-enriched medium, phospho-Nrf2 expression was increased at 1h after TCDD exposure. Expression remained elevated at 24h after TCDD exposure, when H2 had returned to baseline levels. There were also significant increases in HO-1 and NQO-1 expressions . However, at 48h after TCDD exposure, both Nrf2 phosphorylation and HO-1/NQO-1 expression returned to baseline levels. |
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