| External factors: | Rapamycin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//qPCR |
| Description: | This effect on the Nrf2 pathway correlates with inhibition of cell senescence induced by 2-h incubation with H2O2, where our results showed that 24 h of preincubation with rapamycin significantly decreased the levels of p16 and p21 molecular markers,as well as measured by the number of senescent cells measured by β-al staining. |
| Regulatory pathway: | Nrf2//STAT |
| R-EF-Pathway: | Activation//-- |
| Official symbol(s): | NFE2L2//SOAT1 |
| Pathway experiment: | Western blot//qPCR |
| Pathway description: | Pre-incubation of mouse skin fibroblasts with rapamycin for 24 h increased the levels of Nrf2 in a dose-dependent manner, and lowered the levels of Keap1, the cytosolic inhibitor of the Nrf2 pathway. Activation of the Nrf2 pathway is further demonstrated by the levels of Nrf2 in the nuclear localization and by the increase in mRNA levels of down target genes such GST-Ya and NQO1. Our data using lung tissue from Nrf2KO mice showed that tissue from Nrf2KO mice have increased basal levels of p-Stat3, and rapamycin treatment reduced these levels. A similar effect was observed in WI38 cells deficient in Nrf2, which showed increased basal levels of the phosphorylated form of Stat3 (p-Stat3), and rapamycin treatment was able to decrease these levels. |
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