| External factors: | Metformin |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | We observed that 100?μm?metformin effectively stimulated HDF proliferation, which was characterized by the reduced percentage of senescence‐associated β‐galactosidase (SA‐β‐Gal)‐positive cells; 100?μm?metformin also increased the frequency of proliferation‐related KI67‐positive cells . |
| Regulatory pathway: | Nrf2-GPX7//SKN-1-GPX6 |
| R-EF-Pathway: | --//-- |
| Pathway experiment: | Knockdown//Western blot//RNAi |
| Pathway description: | Metformin‐induced GPx7 expression was blocked after NRF2 knockdown ；The positive effect of metformin on stimulating GPx7 expression was dose‐dependent and was confirmed in two independent HDF lines. Moreover, GPx7 expression levels in HDFs were indeed increased throughout the passaging by metformin treatment .skn‐1?RNAi in?C.elegans?completely abrogated the induction of GPX‐6 by metformin, suggesting that metformin upregulates GPX‐6 expression mainly through SKN‐1 in worms. |
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