| External factors: | Cholera toxin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Melanoma |
| Experiment: | BrdU assay |
| Description: | Eventually, exposure to CT-containing medium for up to 12 weeks also resulted in senescence of the lightly pigmented NHM-C melanocytes. |
| Regulatory pathway: | p16-CDK4-pRb |
| R-EF-Pathway: | -- |
| Official symbol(s): | CDKN2A-RB1 |
| Pathway experiment: | Western blot |
| Pathway description: | pRb in NHM-C melanocytes was present in both hypo-and hyperphosphorylated forms through 3 weeks, whereas it was predominantly in the hypophosphorylated form at 6 weeks .In contrast, pRb in NHM-B melanocytes was mostly in the nonphosphorylated form by 3 weeks of CT treatment.At 6 weeks, CDK4-GST-Rb kinase activity was notably reduced in NHM-B as compared to NHM-C cells .Over a 6-week period, levels of CDK4 bound to p16 decreased dramatically in NHM-C melanocytes, whereas it remained almost unchanged in NHM-B cells. |
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