| External factors: | Regulatory T(Treg)cells |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | In contrast, we found significantly increased SA-β-Gal positive T cell populations in na?ve CD4+ T cells after co-culture with CD4+ CD25hiFoxP3+ Treg cells, indicating that Treg cells can induce na?ve CD4+ T cell senescence.?We observed that the percentages of the SA-β-Gal positive cell populations in na?ve CD4+ T cells dramatically increased with longer times of co-culture with CD4+ CD25hiFoxP3+ Treg cells. |
| Regulatory pathway: | p38 MAPK//ERK1/2//TLR8 |
| R-EF-Pathway: | Activation//Activation//-- |
| Pathway experiment: | SA-β-gal activity assay//Western blot |
| Pathway description: | Our transcriptome analyses demonstrated that Treg-induced senescent CD8+ T cells induced significant alterations in genes involved in MAPK signaling pathways. We then confirmed the activation of MAPKs, including ERK1/2, p38 and JNK in na?ve CD4+ T cells treated with CD4+ CD25hiFoxP3+ Treg cells using Western blot analyses. We found that Treg-treated na?ve CD4+ T cells selectively activated ERK1/2 and p38, but not JNK, resulting in significantly enhanced phosphorylation of ERK1/2 and p38. We found that only the TLR8 ligands Poly-G3 and ssRNA40 significantly blocked the induction of responder T cell senescence induced by CD4+ CD25hiFoxP3+ Treg cells identified by SA-β-Gal expression . |
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