| External factors: | Acidic Ph |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Disc degenerative disease |
| Experiment: | Western blot//qPCR |
| Description: | Real-time PCR analysis showed that mRNA expression of senescence-related markers (p16 and p53) in the experimental NP cells was significantly up-regulated compared with the control NP cells. Western blot assay also showed that protein expression of p16 and p53 in the experimental NP cells was increased compared with the control NP cells. |
| Regulatory pathway: | p38 MPAK |
| R-EF-Pathway: | Activation |
| Pathway experiment: | Western blot |
| Pathway description: | Results showed that relative expression of p-p38 MAPK (p-p38/p38) in the experimental NP cells was significantly increased compared with the control NP cells. Reasonably, the inhibitor SB203580 significantly inhibited activation of the p38 MAPK pathway. |
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