| External factors: | Red light |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Western blot//Flow cytometry |
| Description: | Compared with the cells in the control groups, cells in UVA-irradiated groups exhibited significantly increased β-galactosidase activity, suggesting the cells were successfully induced into the aging state (P < 0.05). The UVA plus red light irradiation treatment remarkably improved the aging state of the cells, decreasing the expression of β-galactosidase (P < 0.05).Compared with the expression of SIRT1 in the control groups, there was a significant increase in the UVA-irradiated groups and combined treatment groups (P < 0.05); the combined treatment groups exhibited the highest expression. MMP-1 expression increased in the UVA-exposed groups compared with the control groups (P < 0.05). The combined treatment groups also had a higher expression of MMP-1 compared with the control groups; however, compared with the UVA groups, the expression level was slightly decreased. There was a marked enhancement in the level of p53 acetylation in the UVA-irradiated groups and the combined treatment groups compared with the control groups (P < 0.05), and the red light intervention led to attenuated p53 protein acetylation (P < 0.05). The UVA irradiation resulted in an obvious increasement of p21 and p16 (P < 0.05); and the combined treatment groups conspicuously decreased the expression level of p21 and p16 (P < 0.05).Moreover, the proportion of cells in the red light groups in S phase was also increased, whereas the proportion in G2 phase was decreased compared with the UVAirradiated group. |
| Regulatory pathway: | p38//ERK//JNK |
| R-EF-Pathway: | Downregulation//Downregulation//Downregulation |
| Official symbol(s): | MAPK14//ERK//MAPK8 |
| Pathway experiment: | Western blot |
| Pathway description: | Further results indicated that red light intervention substantially suppressed the expression of p38 and ERK (P < 0.05), whereas the expression of JNK was only slightly decreased.red light intervention significantly suppressed the phosphorylation of p38 and ERK, but had little effect on the phosphorylation of JNK. |
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