Aging Overview

External factors

External factors: UVA
Aging type: Accelerate
Aging characteristic:
Category: Other
Phenotype: Aging
Experimental category: L
Tissue type: Koreskin
Cell name: Fibroblast
PMID: 25039464
Experiment: SA-β-gal activity assay//Western blot//Flow cytometry
Description: Compared with the cells in the control groups, cells in UVA-irradiated groups exhibited significantly increased β-galactosidase activity, suggesting the cells were successfully induced into the aging state (P < 0.05).Compared with the expression of SIRT1 in the control groups, there was a significant increase in the UVA-irradiated groups and combined treatment groups (P < 0.05); the combined treatment groups exhibited the highest expression. MMP-1 expression increased in the UVA-exposed groups compared with the control groups (P < 0.05). The combined treatment groups also had a higher expression of MMP-1 compared with the control groups; however, compared with the UVA groups, the expression level was slightly decreased. There was a marked enhancement in the level of p53 acetylation in the UVA-irradiated groups and the combined treatment groups compared with the control groups (P < 0.05), and the red light intervention led to attenuated p53 protein acetylation (P < 0.05). The UVA irradiation resulted in an obvious increasement of p21 and p16 (P < 0.05); and the combined treatment groups conspicuously decreased the expression level of p21 and p16 (P < 0.05).Compared with nonirradiated control groups, the scale of UVA-irradiated cells in S phase was increased, but not significantly, and the proportion in G2 phase was also increased.


Regulatory relationship

Regulatory pathway: p38//ERK//JNK
R-EF-Pathway: Upregulation//Upregulation//Upregulation
Official symbol(s): MAPK14//ERK//MAPK8
Pathway experiment: Western blot
Pathway description: We measured the protein expression of several molecules that are involved in MAPK signaling and found that UVA irradiation increased the expression of p38、ERK and JNK significantly . Our results showed that UVA irradiation increased the phosphorylation of p38 and ERK and JNK .


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view