| External factors: | Rhus coriaria ethanolic extract |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Breast cancer |
| Experiment: | SA-β-gal activity assay//Cell cycle analysis//Cell morphological analysis |
| Description: | In fact, the G1 population rose from 57% ± 2 in control cells to 67% ± 4 and 71% ± 0.1 in cells treated for 48 h with 200 and 400 μ g/mL RCE, respectively.After 48 h of treating cells with 200 μ g/mL RCE, 21% of cells expressed SA-β-galctosidase; this proportion of senescent cells nearly doubled after 96 h of treatment.A subpopulation of treated cells exhibited a senescence-like phenotype characterized by cell size increase and flattening shape compared to control cells. |
| Regulatory pathway: | p38//ERK1/2 |
| R-EF-Pathway: | Activation//Activation |
| Official symbol(s): | MAPK14// |
| Pathway experiment: | Western blot |
| Pathway description: | Interestingly, a marked induction of phosphorylation of both p38 and ERK1/2 was noted , indicating that these two pathways were activated in response to RCE.Asustained increase of phospho-p38 and phospho-ERK1/2 was observed starting 12?h after RCE treatment and lasted for more than 48?h . |
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