| External factors: | Nutlin-3a |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Lung cancer |
| Experiment: | Clonogenic assays//SA-β-gal activity assay//BrdU Assay |
| Description: | Moreover, clonogenic assays show that a combination of Nut and IR combined treatment leads to a synergistic inhibition of the clonogenic growth of H460 cells. Interestingly, the increased tumor cell killing by IR and Nut combined treatment correlates with the increased expression of senescence biomarkers(increased SA-β-gal staining and decreased BrdU incorporation) in irradiated lung cancer cells. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Our data demonstrate that Nut treatment substantially increases IR-induced p53 activation and p21 expression in H460 cells. |
Annotation: