Aging Overview

External factors

External factors: Nutlin-3a
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Lung cancer
Experimental category: L
Tissue type: --
Cell name: NSCLC,H460
PMID: 23683497
Experiment: Clonogenic assays//SA-β-gal activity assay//BrdU Assay
Description: Moreover, clonogenic assays show that a combination of Nut and IR combined treatment leads to a synergistic inhibition of the clonogenic growth of H460 cells. Interestingly, the increased tumor cell killing by IR and Nut combined treatment correlates with the increased expression of senescence biomarkers(increased SA-β-gal staining and decreased BrdU incorporation) in irradiated lung cancer cells.


Regulatory relationship

Regulatory pathway: p53
R-EF-Pathway: Activation
Official symbol(s): TP53
Pathway experiment: Western blot
Pathway description: Our data demonstrate that Nut treatment substantially increases IR-induced p53 activation and p21 expression in H460 cells.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view