| External factors: | Nutlin-3a |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Cutaneous T-Cell Lymphoma |
| Experimental category: | L |
| Tissue type: | Plaque,Blood,Lymphoma |
| Cell name: | MyLa2000,SeAx,HuT 78,Mac2a |
| PMID: | 22377766 |
| Experiment: | BrdU Assay//Flow cytometry//SA-β-gal activity assay |
| Description: | Exposure to nutlin induced a complete inhibition of BrdU incorporation in MyLa2000, Mac1, and Mac2a within 24 hours. Flow cytometric cell-cycle profiling further demonstrated an 80% reduction in the percentageof cells in the S phase, with a concomitant increase in the G0/G1 population.MyLa2000 and Mac2a cells were clearly SA-β-gal positive after 72 hours of exposure to nutlin-3a. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | The p53 protein accumulation after nutlin-3a was effectively downregulated in p53 siRNA– transfected groups compared with the scrambled siRNA– transfected ones . |
Annotation: