| External factors: | Nutlin-3a |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | SA-β-gal activity assay markedly increased in size, acquired flat morphology, and showed intense SA-β-Gal staining typical for senescent cells .In cubation of the lung cancer cell line H460 with 10 μmol/L nutlin-3a, the Activate enantiomer of nutlin-3, for 24 hours led to an effective cell cycle arrest primarily in G1 phase. |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Knockdown//BrdU assay |
| Pathway description: | Knockdown of p53 by specific siRNA that reduced p53 protein levels >85% compared with siRNA controls (data not shown) completely abolished nutlin-induced cell cycle arrest, confirming that it is driven by p53 activation. |
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