| External factors: | Nutlin-3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Cell Cycle analysis |
| Description: | All cells stained intensely for SA-β-Gal activity .Bromodeoxyuridine (BrdU) labeling revealed that less than 1% of the cell population is in S phase, indicating that they have exited the cell cycle . |
| Regulatory pathway: | p53 |
| R-EF-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Treatment of early passage and senescent WI-38 cells with 10 μM nutlin-3a for 24 hours elevated p53 protein in both senescent and early passage cells . |
Annotation: