| External factors: | Wharton’s jelly extract |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay |
| Description: | In the middle-phase (30 PD) of culturing, cells on uncoated plates began to change to a flattened and enlarged phenotype, while cells on the WJE-coated plates remained fibroblast-like.In the latephase (50 PD) of culturing, cells on uncoated plates exhibited a flat and hypertrophic phenotype, while most cells on WJE-coated plates remained spindle-shaped.All three samples of UC-MSCs cultured on uncoated plates showed a significant increase in the percentage of SA-β-gal-positive staining with passaging, while the proportion of senescent UC-MSCs cultured on WJE-coated plates increased slowly.Furthermore, for BMMSCs plated on WJE-coated plates, SA-b-gal positive cells were effectively reduced until 30 PD,then positive cells gradually increased (P,0.01, n = 3). |
| Regulatory pathway: | p53//p16-pRb |
| R-EF-Pathway: | Downregulation//Downregulation |
| Official symbol(s): | TP53// |
| Pathway experiment: | Western blot |
| Pathway description: | The expression of p53, p16INK4a, and pRb in UC-MSCs cultured on WJE-coated plates was decreased at 30 PD, 50 PD compared to cells cultured on uncoated plates.We concluded that the WJE-coated surface provides an ideal environment that efficiently suppresses p53 and p16INK4a/pRb expression in UC-MSCs to delay replicative senescence of MSCs. |
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