| External factors: | Simvastatin |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Chemical compounds |
| Phenotype: | Melanoma |
| Experiment: | SA-β-gal activity assay//PI staining//Flow cytometry |
| Description: | Our results show that simvastatin-treated WM9 cells developed some aspects of cellular senescence, including increased senescence-associated β-galactosidase activity and a G1/S cell cycle arrest. |
| Regulatory pathway: | p53//p21 |
| R-EF-Pathway: | Upregulation//Upregulation |
| Official symbol(s): | TP53//CDKN1A |
| Pathway experiment: | Western blot//qRT-PCR |
| Pathway description: | The p53 and p21 mRNA levels were significantly increased after treatment of WM9 cells with simvastatin at the concentrations of 0.25 and 1 μmol/L .We observed an increased expression of phospho-p53 and p21 protein levels in WM9 cells treated with simvastatin , which are in agreement with RT-qPCR data . |
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