| External factors: | Ionizing radiation |
| Aging type: | Accelerate |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Lung cancer |
| Experiment: | Western blot//SA-β-gal activity assay//BrdU Assay |
| Description: | The results reveal a substantial increase in SA-β-gal positive senescent cells in irradiated lung cancer cells.Similar results were also observed in A549 cells. Moreover, high levels of p16 expression, another important biomarker of senescence [25], were detected in irradiated H460 cells. In addition, BrdU incorporation assays show that the senescent lung cancer cells are unable to synthesize DNA and incorporate BrdU. |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | Our findings here demonstrate that IR activates the p53–p21 pathway in a dose-dependent manner as evidenced by increased expression of phosphorylated p53 and p21 in irradiated H460 cells. |
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