| External factors: | LMWP-SOD1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Category: | Other |
| Phenotype: | Aging |
| Experiment: | SA-β-gal activity assay//Flow cytometry//MTT assay |
| Description: | Control DPSCs (+H2 O2 ) and SOD1-treated DPSCs (+H2 O2 ) were 28.5% and 26.8% positive, respectively, for SA-β-gal staining ; however, LMWP-SOD1-treated DPSCs (+H2 O2 ) were 12.3% positive for SA-β-gal staining. LMWP-SOD1 slightly restored cell viability which had been inhibited by H2 O2. Similarly, for the control DPSCs (+H2 O2 ) and the SOD1-treated DPSCs (+H2 O2 ), the number of cells in G1 phase was increased, but the number of LMWP-SOD1-treated DPSCs (+H2 O2 ) in G1 phase slightly and significantly decreased compared with control DPSCs (+H2 O2 ) and SOD1-treated DPSCs . |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot//RT-PCR |
| Pathway description: | The control DPSCs (+H2 O2 ) and SOD1-treated DPSCs (+H2 O2 ) had no influence on mRNA expression, but LMWP-SOD1- treated DPSCs (+H2 O2 ) significantly abolished the H2 Oinduced increase in p53 and p21Cip1/WAF1 mRNA expression levels to the basal state. The p53 and p21Cip1/WAF1 protein levels were also quantified by Western blot analysis. As with the mRNA levels, treatment with LMWP-SOD1 partially abolished H2 O2 -induced p53 and p21Cip1/WAF1 protein expression in human DPSCs. |
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