| External factors: | 5AZA-dC |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Category: | Chemical compounds |
| Phenotype: | Tumor |
| Experiment: | Western blot//Cell morphological analysis//SA-β-gal activity assay//Cell counting |
| Description: | 5AZA-dC-treated cells showed the expression of p16INK4A, enlarged cell size, growth arrest, and senescence associated β-gal staining. |
| Regulatory pathway: | p53-p21 |
| R-EF-Pathway: | Activation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | 5AZA-dC-treated cells indeed showed upregulation of p53 and its downstream effector, p21WAF1, in addition to p16INK4a, and exhibited nuclear translocation of the p53 protein. Whereas only 10% 20% of control cells showed nuclear p53, 90% of the 5AZA-dC-treated cells showed strong p53 staining in the nucleus. |
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